New research improves accuracy of molecular quantification in high throughput sequencing

February 09, 2024

Accurate sequencing of genetic material is crucial in modern biology, particularly for comprehending and addressing diseases linked to genetic anomalies. While UMIs have been widely adopted in sequencing methods, the study reveals that PCR errors can undermine the accuracy of molecular quantification, particularly across different sequencing platforms. Jianfeng explained: 'PCR amplification, essential for most RNA sequencing techniques, can introduce errors, compromising data integrity. By rectifying PCR errors in UMIs, it greatly boosts molecular quantification accuracy in various sequencing applications. The paper, 'Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules', can be read in Nature Methods.

The source of this news is from University of Oxford