PhD Position in New Single-Molecule Proteomics Methods

Department of Chemistry

60 %

The Laboratory of Chemical and Biological Probes (Prof. Rivera-Fuentes) is looking for a PhD student to work on the development of a new method for single-molecule protein identification. We recently demonstrated a new method (called “blinkognition”) to identify peptides with single-molecule resolution. You can learn about this method by reading our recent paper and also by watching this simple explainer video. The goal of the project is to extend this technique to full-length, folded proteins.

As a PhD student working on this project, you would express and purify proteins, label them using a variety of bioconjugation strategies, immobilize them onto a glass surface, perform single-molecule fluorescence measurements using a TIRF microscope, and analyze their traces using our recently developed deep-learning model. You would also develop new analysis pipelines in collaboration with a machine learning specialist in our lab. Additionally, you would be an active member of our group, contributing to routine lab maintenance, participating in group meetings and in selected outreach activities. You would also contribute to teaching as assistant in lab courses and lectures. You would also write reports and papers, and prepare presentations for national and international conferences.

Highly motivated student with a solid background in chemistry, biochemistry, or biophysics. Strongly interested in developing new technologies to analyze proteins with single-molecule resolution and revolutionize the field of proteomics. Previous experience in protein chemistry, single-molecule imaging, and/or machine learning is an advantage. MSc degree in chemistry or biochemistry and proficiency in oral and written English are formal requirements.

We are a dynamic, interdisciplinary, medium-size lab working at the interface of chemistry and biology. We work on the development of probes to visualize and control biological processes in living systems. World-class infrastructure is available in our lab, the department, or at technology platforms of the University of Zurich. We are located at University of Zurich, Irchel campus, where you would work in one of the newest and most modern chemistry buildings in Europe.

University of Zurich Irchel, Winterthurerstrasse 190, 8057 Zürich, Switzerland

Employment start date to be mutually agreed. Please submit your application as a single PDF file including a motivation letter, CV and copy of transcripts. Letters of recommendation will only be required for shortlisted candidates. Applications will be received until February 25, 2023. Incomplete applications or those arriving after the deadline will not be considered.