- Molecular Biology (cloning, PCRs, radioactive labeling analyses, extractions, and analyzes of nucleic acids and proteins) - Cell Biology (optical and fluorescence imaging) - Genetics of Saccharomyces cerevisiae (CRISPR/Cas9 genome modifications; transformations; crosses; use of gene expression control tools) - Microfluidics (analysis of replicative senescence in individual lines in circuits specifically developed for our projects) - Bio-informatics analysis (high throughput sequencing)
- Characterize the effects of telomerase inactivation and telomere shortening on other cellular components and compartments.
PI: Maria Teresa Teixeira (firstname.lastname@example.org) Projects in the team “Telomere Biology” led by MT Teixeira target the comprehensive dissection of the pathways causing replicative senescence induced by telomere length erosion and the understanding of their impact on proliferative potential. Our strategy is to decompose the sources of cell-to- cell variations inherent to telomere and senescence to uncover the molecular basis of telomere control over cell proliferation limit. We thus analyze telomeres at the scale of single molecules and replicative senescence at the level of single-cell lineages. We combine the power of budding yeast genetics and genomics with state-of-the-art cell imaging techniques, microfluidics, quantitative analysis, and mathematical modeling. H. Martin, M. Doumic, M. T. Teixeira and Z. Xu (2021). Cell Biosci 11(1): 180. Telomere shortening causes distinct cell division regimes during replicative senescence in Saccharomyces cerevisiae. H. Coutelier, et al. (2018). Genes Dev 32(23-24): 1499-1513. Adaptation to DNA damage checkpoint in senescent telomerase-negative cells promotes genome instability. Z. Xu, E. Fallet, C. Paoletti, S. Fehrmann, G. Charvin and M. T. Teixeira (2015). Nature Comm 6: 7680. Two routes to senescence were revealed by real- time analysis of telomerase-negative single lineages. J. Soudet, P. Jolivet and M. T. Teixeira (2014). Mol Cell 53(6): 954-964. Elucidation of the DNA end-replication problem in Saccharomyces cerevisiae.
All the experiments will be carried out at the CNRS, at the Institute of Physico-Chemical Biology on the Curie campus, in the 5th arrondissement of Paris. All the necessary equipment is already in the laboratory or within a very close radius.Eligibility criteria
Skills: - Expert in Molecular and Cellular Biology - Expert in Genetics - General knowledge in Bio-Informatics - Fluent in English, good skill in scientific communication
Know-how - Design and perform experiments in Molecular and Cellular Biology and Genetics - Plan and implement a scientific study - Organize your work on a daily basis - Keeping a laboratory notebook and presenting your results
Soft skills: - Work in a team - Open-minded, curious and good at synthesising information - Organised, like to do a good job and finish it well - critical of results, honest
Management skills: - Co-supervision of Master students possibleAdditional comments
The application file must be written in English and submitted on the site https: // emploi.cnrs.fr/. It should include a detailed CV, a minimum of two reference letters, a cover letter outlining your short and long-term career goals, and a signed statement that you comply with the mobility rule which states that you have not resided or exercised your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. The complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at the email address DYNAMOcofund@ibpc.fr. See details on the website https:// labexdynamo.ibpc.fr/fp-dynamo-paris/
Evaluation The position is designed as a first step in a research career, and the assessment of applicants will be primarily based on their research qualifications and skills that can complement and reinforce ongoing research at partner institutions.
Step 1. Applications from each eligible candidate will be assessed by independent international experts on the relevant research topic. Eligible applicants will be assessed on merit, motivation for the program, academic excellence, and personal qualifications such as their ability to collaborate and communicate.
Step 2. A maximum of three candidates will be selected for a video interview with independent experts for each open position. After the interview, shortlisted candidates will be invited to a remote conference with the project manager and a member of the DYNAMO board of directors to define the general scope of the project, the training, and the work environment. research. Applicants will be required to write a 3-page project proposal, bringing their original ideas, and will have 2 weeks to submit it.
Step 3. Each project proposal will be evaluated by one of the independent experts and a member of the DYNAMO board of directors who will have evaluated the applicant during the previous stages. The final selection will be made based on the marks obtained in stages 1, 2, and 3. The minimum mark must be greater than 20/40. The best candidate will be offered the postdoctoral position with a start date of January 1, 2023.
The CNRS (National Center for Scientific Research) is an employer subscribing to the principle of equal access to employment. Furthermore, FP- DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages them to apply.Web site for additional job details
https: // emploi.cnrs.fr/Offres/CDD/FR550-MAGTOR-016/Default.aspxRequired Research Experiences
Biological sciences › Biology
Biological sciences: PhD or equivalent
FRENCH: BasicContact Information