- Molecular Biology (cloning, PCR, RNA, and protein preparation and analysis) - Yeast genetics (mutation, transformation, crossing, tools for controlling gene expression) - Analysis of RNA-protein complexes
- Characterizing translation-coupled RNA decay and protein quality control factors
PI: Lionel BENARD (email@example.com) Our team is investigating cellular processes that identify and degrade faulty mRNAs and their corresponding aberrant peptides, potentially toxic to cells. Ribosome-based quality controls have received much attention during the past decade, leading to the characterization of highly conserved surveillance effectors that rescue stalled ribosomes and degrade aberrant/damaged mRNAs and truncated peptides. Studies also suggest that alternative processes exist when these factors cannot operate, in stress conditions, in particular, involving mechanisms and factors of quality control that we aim to characterize, using S. cerevisiae as a model organism. The project combines yeast genetics, molecular biology and RNA biology, protein tagging, and immunoprecipitation including proteomics. We aim to determine the molecular composition of complexes critical in Ribosome Quality Control and define the roles of newly identified protein factors.
Representative publications of the Group: -Navickas, A., Chamois, S., Saint-Fort, R., Henri, J., Torchet, C., Benard, L. (2020). No-Go Decay mRNA cleavage in the ribosome exit tunnel produces 5'-OH ends phosphorylated by Trl1. Nature communications 11: 122. -Dhaliwal, J.S., Panozzo, C., Benard, L., Zerges, W. (2020). Oxidized RNA Bodies compartmentalize translation quality control in Saccharomyces cerevisiae. Pre-print in BioRxiv. DOI: https: // doi.org/10.1101/2020.08.05.232983. -Gilet, J., Conte, R., Torchet, C., Benard, L., Lafontaine, I. (2020). An additional layer of regulation via convergent gene orientation in yeasts. Molecular biology and evolution. Vol. 37, Issue 2, Pages 365–378.
All the experiments will be carried out at the CNRS, at the Institute of Physico-Chemical Biology on the Curie campus, in the 5th arrondissement of Paris. All the necessary equipment is in the laboratory or in a very close radius within the framework of collaborations which could be set up.Eligibility criteria
Knowledge: - Expert in Molecular Biology - Good knowledge of RNA Biology - Knowledge in Yeast genetics would be appreciated - Experience with proteomics and high-throughput sequencing would be an advantage. - Fluent in English, good skills in scientific communication
Know-how - Design and perform experiments - Plan and implement a scientific study - Organize your work daily - Keeping a laboratory notebook and presenting your results
Soft skills - Good humor, friendly, and plenty of energy - Team oriented - A passion for science and learning, good at synthesizing information - Organised, like to do a good job, and critical of results
Managements kills - Co-supervision of Master students possibleAdditional comments
The application should be written in English and submitted to https: // emploi.cnrs.fr/. It must include a detailed CV, a minimum of two letters of reference, a cover letter describing your short and long-term career objectives, and a signed declaration that you comply with the mobility rule which stipulates that you have not resided or carried out your main activity (work or studies) in France for more than 12 months during the three years preceding the date of recruitment. The complete file must also be sent to Bruno MIROUX & Magdalena TORTYNA, respectively director and manager of the project at DYNAMOcofund@ibpc.fr. See details on the website https:// labexdynamo.ibpc.fr/fp-dynamo-paris/
Evaluation The position is designed as a first step in a research career, and the evaluation of candidates will be based primarily on their research qualifications and skills that can complement and strengthen ongoing research at the partner institutions. Step 1. The applications of each eligible candidate will be evaluated by independent international experts within the relevant research theme. Eligible candidates will be evaluated based on merit, motivation for the program, academic excellence, and personal qualifications such as collaboration and communication skills. Step 2. Up to three candidates will be selected for a video interview with the independent experts for each open position. After the interview, short-listed candidates will be invited to a remote conference with the project director and a DYNAMO board member to define the general scope of the project, training, and research environment. Applicants will be asked to write a 3-page project proposal, contributing their original ideas, and will have 2 weeks to submit it. Step 3. Each project proposal will be evaluated by one of the independent experts and the DYNAMO board member who evaluated the applicant in the previous steps. Final selection will be made based on the scores obtained in Steps 1, 2, and 3. The minimum score must be above 20/40. The best candidate will be offered the postdoctoral position with a start date of January 1 to April 30, 2023.
The CNRS (Centre National de la Recherche Scientifique) is an equal opportunity employer. In addition, FP-DYNAMO-PARIS is committed to promoting the role of women in science and thus explicitly encourages them to apply.Web site for additional job details
https: // emploi.cnrs.fr/Offres/CDD/FR550-MAGTOR-015/Default.aspxRequired Research Experiences
Chemistry › Biochemistry
Physics › Biophysics
Biological sciences › Biological engineering
Chemistry: PhD or equivalent
Physics: PhD or equivalent
Biological sciences: PhD or equivalent
FRENCH: BasicContact Information