-Cell culture of human iPSC and differentiation in motor neurons. -RNA-seq analysis to identify pertinent targets. -CRISPR-Cas9 editing of gene of interest for live imaging of local translation. -Coordinate experiments between all partners of the project. -Train and supervise doctoral candidates, trainees or young researcher that are present at our lab for short mobilities. -Writing of scientific report and articles. -Writing of grant proposals.
The post-doctoral researcher will work in the sub-group “understanding the molecular mechanism of SMA” at the IGMM with the aim to identify mislocalized axonal mRNA in SMA motor neurons and study their dynamics of local translation in healthy and pathological conditions. He/she will have to perform cell culture of motor neurons derived from iPSCs in microfluidic devices, use and optimize molecular biology techniques as well as live cell imaging in order to biophysically model mRNA transport and translation in SMA motor neurons. This contract is part of an interdisciplinary project in collaboration with a team in Israel that will perform the same missions as the post-doctoral researcher to study another neurodegenerative disease.
The project will be done at IGMM (Institute of Molecular Genetic of Montpellier). The institute is renown internationally, particularly in the research area of mRNA functions and mechanisms. Moreover, it hosts the iPSC- neurons unit from the Platform Organoides of Montpellier (POM), where iPSC differentiation in motor neurons, as well as CRISPR-Cas9 editing is mastered. The institute also benefit from a lot of equipment that allow the use of interdisciplinary approaches required for this project. IGMM is located on a campus with an access to MRI platform that possesses a large collection of microscopes crucial for the development of this project.Eligibility criteria
•Knowledge in Biology, Neuroscience et laboratory techniques. •Master molecular biology techniques •Master cell culture (knowledge in iPSC and neuron culture is an added value) •Master live cell imaging with the use and confocal microscopy •Area of education favored: Neuroscience and Health/Biology •Good English level (both speaking and writing) •Good writing level and ability to synthesis ideas •Have experience of leading meetings •Have experience of writing grant proposals •Good organization and management skills required A PhD in biology or a similarly applied domain is required.Web site for additional job details
https: // emploi.cnrs.fr/Offres/CDD/UMR5535-SARADE-023/Default.aspxRequired Research Experiences
Neurosciences: PhD or equivalent
Biological sciences: PhD or equivalent
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