Phd Studentship In Structure/Function Of L,D-Tpase Involved In The Integrity Of The Bacterial Envelope For In Silico Antimicrobial Design Within The European Consortium Breakthrough.
Universities and Institutes of France
17 May 2023
Job InformationOrganisation/Company
CNRS-IBS
Department
UMR5075
Research Field
Biological sciences » Biology
Researcher Profile
First Stage Researcher (R1)
Country
France
Application Deadline
1 Jul 2023 - 10:00 (Europe/Madrid)
Type of Contract
Temporary
Job Status
Full-time
Hours Per Week
35
Offer Starting Date
1 Sep 2023
Is the job funded through the EU Research Framework Programme?
HE / MSCA
Reference Number
101072632
Marie Curie Grant Agreement Number
101072632
Is the Job related to staff position within a Research Infrastructure?
No
Offer DescriptionThe objective of this project is to resolve the structure/activity relationships of Lpp binding to the bacterial cell wall by LdtA,B,C transpeptidases. Cells that lack it have greater membrane permeability and sensitivity to antibiotics due to increased permeability of their outer membrane. Therefore, targeting the binding of the Lpp to the peptidoglycan layer should eliminate bacteria in patients.
Activities
Study the structure of different Ldt and their interaction with peptidoglycan and Lpp using NMR and/or X-ray crystallography. These structural studies will be complemented by biochemical and biophysical analysis of the characteristics of Ldt/peptidoglycan/Lpp interactions. The observations made will be validated in vivo at the level of the BREAKthrough consortium. In parallel, molecular docking studies will be carried out with substrates and inhibitors and different biophysical methods will be used to characterize the interaction between Ldt and inhibitors synthesized by other groups of the BREAKthrough consortium. As part of this thesis, 6 months will be dedicated to scientific stays in three public and private laboratories of the consortium.
RequirementsResearch Field
Biological sciences » Biology
Education Level
Master Degree or equivalent
Research Field
Chemistry » Biochemistry
Education Level
Master Degree or equivalent
Research Field
Chemistry » Physical chemistry
Education Level
Master Degree or equivalent
Skills/Qualifications
-Notions in Microbiology
- Knowledge in Structural Biology
- Knowledge of Nuclear Magnetic Resonance(NMR)
- Expertise in Purification and protein characterization.
- Master's degree in chemistry, biology or pharmacy
- English (written/oral) level C1 spoken and written.
Specific Requirements
To be eligible to apply for this position as part of the European BREAKthrough project, candidates must meet the following criteria.
- You have not yet obtained (or successfully defended) a PhD.
- You have not resided or carried out your main activity (work, studies, etc.) in the country of the institution recruiting you for more than 12 months during the 36 months immediately preceding your date of entry into service. Compulsory national service, short-term stays such as holidays and time spent in a procedure for obtaining refugee status are not taken into account.
- The research will be done indifferently in French or English, however it is expected that the non-French-speaking candidate strives to learn the basics of French in order to facilitate communication and its integration into the laboratory.
Languages
ENGLISH
Level
Good
Internal Application form(s) needed
CNRSProfil CDD DoctorantITN BreakthoughRMN2023JPSCLENG-1.pdf
English
(106.48 KB - PDF)
Download
Additional InformationBenefits
Work context
Located on the Polygone scientifique de Grenoble (ESRF/ILL/EMBL/IBS), the Institute of Structural Biology (IBS) is a national and international player in the field of integrated structural biology. At the same time research center, technical platform, host site and scientific training, the IBS aims to develop research in structural biology, a field of research crucial for the understanding of fundamental biological mechanisms. It is based on 12 state- of-the-art platforms. As a joint research unit (CEA-CNRS-UGA) IBS is composed of 19 research groups, each of these groups proposing a multidisciplinary approach, at the interface of biology, physics and chemistry, in coherence with 3 research axes. Nearly 300 people work at IBS, researchers, doctoral students, engineers and technicians in a multicultural and international environment. The person recruited will join the NMR group that works on the bacterial cell wall.
Eligibility criteria
To be eligible to apply for this position as part of the European BREAKthrough project, candidates must meet the following criteria.
- You have not yet obtained (or successfully defended) a PhD.
- You have not resided or carried out your main activity (work, studies, etc.) in the country of the institution recruiting you for more than 12 months during the 36 months immediately preceding your date of entry into service. Compulsory national service, short-term stays such as holidays and time spent in a procedure for obtaining refugee status are not taken into account.
- The research will be done indifferently in French or English, however it is expected that the non-French-speaking candidate strives to learn the basics of French in order to facilitate communication and its integration into the laboratory.
Additional comments
General information
Type of contract: fixed-term
Section : 20
Typical job: PhD student
Contract duration: 36 months
Expected hiring date: 01/09/2023
Work quota: Full-time
Desired level of study: Master 2
Desired experience: 0 to 5 years
Gross salary: between 2945 € and 3364 € (depending on family situation)
Work Location(s)Number of offers available
1
Company/Institute
CNRS IBS
Country
France
City
Grenoble
Postal Code
38044
Street
71, avenue des Martyrs
Geofield
Where to applyCity
Grenoble
Website
https:// www. ibs.fr/
Street
71, avenue des Martyrs
Postal Code
38044
STATUS: EXPIRED