Phd Protéins Biochemsitry M/F

Universities and Institutes of France
November 14, 2022
Contact:N/A
Offerd Salary:Negotiation
Location:N/A
Working address:N/A
Contract Type:Temporary
Working Time:Full time
Working type:N/A
Job Ref.:N/A
  • Organisation/Company: CNRS
  • Research Field: Biological sciences › Biological engineering Chemistry › Biochemistry Physics › Biophysics
  • Researcher Profile: First Stage Researcher (R1)
  • Application Deadline: 14/11/2022 23:59 - Europe/Brussels
  • Location: France › GIF SUR YVETTE
  • Type Of Contract: Temporary
  • Job Status: Full-time
  • Hours Per Week: 35
  • Offer Starting Date: 01/12/2022
  • The project will be achieved at the Institute of Integrative Cell Biology (I2BC), a research institute 20 km south-west from Paris (direct commuting with Paris downtown in 35 minutes). The host team (PROMTI) is interested in modifications of the N-terminus of proteins. Among these modifications, N-myristoylation and N-acetylation are two modifications carried out in competition. The team aims at better understanding the functional elements and certain physiological conditions that favor one or other of these modifications.

    This thesis is dedicated to the understanding of the mechanism of action of IpaJ on its specific targets in human cells. This encompasses many myristoylated proteins. The study will address the issue of the impact of the expression of the modifiers that may compete to the myristoylation site of the targets, basically NMT and NatA. The focus will be first carried out on the Shigella flexneri version of IpaJ. There are actually IpaJ homologs in many pathogenic bacteria. Such IpaJ homologs have never been characterized and it is not known which their respective specificity is. If the setting of the system is working well, the Salmonella enterica will be studied. Pathogenic S. enterica serotypes exhibit indeed an IpaJ homolog (75% homology) which causes similar physiological effects as those triggered by the S. flexneri toxin 10. Gaining information from either IpaJ variants should help better understanding of IpaJ targeting. Investigation of both N-MYR and Ꜫ-MYR will be achieved at the level proteome of the entire proteome using novel click chemistry approaches to get relevant information. Classic and novel inhibitors of NMT will be used. Three Work Packages (WPs) define the goals of the research that will be performed.

    Web site for additional job details

    https: // emploi.cnrs.fr/Offres/Doctorant/UMR9198-THIMEI1-004/Default.aspx

    Required Research Experiences
  • RESEARCH FIELD
  • Chemistry › Biochemistry

  • YEARS OF RESEARCH EXPERIENCE
  • None

  • RESEARCH FIELD
  • Physics › Biophysics

  • YEARS OF RESEARCH EXPERIENCE
  • None

  • RESEARCH FIELD
  • Biological sciences › Biological engineering

  • YEARS OF RESEARCH EXPERIENCE
  • None

    Offer Requirements
  • REQUIRED EDUCATION LEVEL
  • Chemistry: Master Degree or equivalent

    Physics: Master Degree or equivalent

    Biological sciences: Master Degree or equivalent

  • REQUIRED LANGUAGES
  • FRENCH: Basic

    Contact Information
  • Organisation/Company: CNRS
  • Department: Institut de Biologie Intégrative de la Cellule
  • Organisation Type: Public Research Institution
  • Website: https:// www. i2bc.paris-saclay.fr
  • Country: France
  • City: GIF SUR YVETTE
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