Phd Position: Cryo-Em Structures Of Human Translation Complexes

4 Aug 2023

Job Information

Organisation/Company

CERBM

Department

CBI

Research Field

Biological sciences » Biology

Researcher Profile

First Stage Researcher (R1)

Country

France

Application Deadline

20 Dec 2023 - 12:00 (Europe/Paris)

Type of Contract

Permanent

Job Status

Full-time

Offer Starting Date

1 Jan 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

PhD position: cryo-EM structures of human translation complexes

The Klaholz lab focuses on the molecular mechanism of translation by the human ribosome and its regulation. Using different sample preparation methods we have optimized the procedure of human ribosome purification for high- resolution studies (Khatter et al. Nature 2015; Frechin et al. JSB 2022). Building on these achievements, we seek to recruit a highly motivated doctoral student (PhD student) that will work on cryo-EM structures of human translation complexes. The project benefits from state-of-the art cryo electron microscopy instrumentation installed at the CBI/IGBMC which hosts French and European infrastructures.

The successful candidate will work on a cutting-edge experimental research project to obtain a high resolution structures involved in translation using the latest generation technology for cryo-EM data collection and image analysis. The project involves sample preparation high-resolution cryo-EM data collection, image processing, atomic model building and structure interpretation as well as functional analysis of human translation via biochemical assays in vitro and in vivo.

The project will be carried out under the supervision of Ottilie von Loeffelholz in the friendly and supportive atmosphere of the Klaholz group, which has a strong track record in ribosome structure determination. The successful candidate will benefit from the thriving scientific environment of the CBI/IGBMC hosting a state-of-the-art cryo-EM facility including a Glacios equipped with a K2 camera, a G2 Titan Krios equipped with Volta phase plate, Gatan energy filter and Falcon III and K3 direct electron detectors as well as the recently installed G4 Titan Krios equipped with Volta phase plate, Selectris X energy filter and Falcon IVi direct electron detector. In addition the candidate will have access to the most recent equipment and services for biochemical sample preparation ranging from platforms for human cell cultures, molecular cloning, biophysical sample characterisation, genome-wide sequencing, and the possibilities to work with in vivo models. Highly performant in-house computational resources are available for the group as well as additional computational resources at the centre de calcul de l'Université de Strasbourg.

Please send your application including a statement describing research experience and expertise as well as future research interests, a curriculum vitae with publication list, date of availability, and at least two contact details for references (including phone number and email) to Dr Ottilie von Loeffelholz ( [email protected]) or Dr Bruno Klaholz ([email protected]). Informal enquires are very much welcome.

Selected Articles:

Fréchin, L., Holvec, S., von Loeffelholz, O., Hazemann, I., and Klaholz, B.P. (2022). High-resolution cryo-EM performance comparison of two latest- generation cryo electron microscopes on the human ribosome. J. Struct. Biol., 107905. 10.1016/j.jsb.2022.107905.

Khatter, H., Myasnikov, A.G., Natchiar, S.K., and Klaholz, B.P. (2015). Structure of the human 80S ribosome. Nature 520 , 640–645. 10.1038/nature14427.

von Loeffelholz, O., Natchiar, S.K., Djabeur, N., Myasnikov, A.G., Kratzat, H., Ménétret, J.-F., Hazemann, I., and Klaholz, B.P. (2017). Focused classification and refinement in high-resolution cryo-EM structural analysis of ribosome complexes. Curr. Opin. Struct. Biol. 46 , 140–148. 10.1016/j.sbi.2017.07.007.

Bhaskar, V., Graff-Meyer, A., Schenk, A.D., Cavadini, S., von Loeffelholz, O., Natchiar, S.K., Artus-Revel, C.G., Hotz, H.-R., Bretones, G., Klaholz, B.P., et al. (2020). Dynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes. Cell Rep. 31 , 107473. 10.1016/j.celrep.2020.03.037.

Requirements

Research Field

Biological sciences » Biology

Education Level

Master Degree or equivalent

Skills/Qualifications

Eductaion in biology, biochemistry, chemistry or structural biology

Specific Requirements

Konwledge in some of the listed topic is essetial: protein production, atomic model building, structure determination, biochemical assays, working with proteins, working with RNA, ultracentrifugatio.

Basic knowledge in linux, electron microscopy, crystallography, cryo-EM are an advantage.

Languages

ENGLISH

Level

Good

Additional Information

Benefits

fully funded PhD

Website for additional job details

https: // www. igbmc.fr/equipes/grands-complexes-impliques-dans-lexpression-des- ge…

Work Location(s)

Number of offers available

1

Company/Institute

IGBMC/CBI

Country

France

State/Province

Alsace

City

Illkirch

Postal Code

67404

Street

rue laurent fries

Geofield

Where to apply

E-mail

[email protected]

Contact

City

Illkirch (Strasbourg)

Website

https:// www. igbmc.fr/

Street

rue Laurent Fries 1

Postal Code

67404

E-Mail

[email protected]

STATUS: EXPIRED