M/F Phd Fellowship Dynamic Control In Hybrid Plasmonic Nanopores: Road To Next Generation...

Universities and Institutes of France
September 21, 2022
Offerd Salary:Negotiation
Working address:N/A
Contract Type:Temporary
Working Time:Full time
Working type:N/A
Job Ref.:N/A
  • Organisation/Company: CNRS
  • Research Field: Engineering Physics Technology
  • Researcher Profile: First Stage Researcher (R1)
  • Application Deadline: 21/09/2022 23:59 - Europe/Brussels
  • Location: France › MARSEILLE 13
  • Type Of Contract: Temporary
  • Job Status: Full-time
  • Hours Per Week: 35
  • Offer Starting Date: 01/01/2023
  • Eu Research Framework Programme: H2020 / Marie Skłodowska-Curie Actions
  • The Institut Fresnel is a research state laboratory based in Marseille / France, devoted to research and higher education with affiliation to both CNRS and Aix Marseille University. Institut Fresnel is seeking to recruit talented, enthusiastic young scientists who are highly motivated to boost their research career in the areas of nanosciences, biosensing, and biotechnologies.

    The successful candidate will be part of an emerging project carried out at the Institut Fresnel under the supervision of Jérome Wenger with the funding of Marie Sklodowska-Curie Doctoral Networks HORIZON-MSCA-DN-2021 project called DYNAMO. DYNAMO is designed as an innovative and pioneering training network, with the unique vision of developing the next-generation hybrid nanopore technology exploiting DNA nanostructures integrated with multifunctional solid-state platforms

    Jerome Wenger's group has acquired a wide expertise in the nanoscale control of light fields in plasmonic nanostructures and its application to enhance fluorescence spectroscopy applications. For our next project within the DYNAMO consortium, we will focus on plasmonic trapping and enhanced UV label-free single protein detection with plasmonic nanopores. Objectives: The aim of this project will be to exploit the intrinsic UV autofluorescence of proteins (due to their natural aromatic aminoacids) to achieve single molecule detection in a label-free manner. The JR will develop a nanopore platform able to trap single entities by means of magnetic force and/or in combination with plasmonic nano-optical trapping using the red laser. The spectroscopy approach will be combined with dedicated aluminium nanopores to (i) enhance the deep-UV autofluorescence signal, (ii) monitor translocation and (iii) screen the background. To investigate possible conformational changes of the protein, the JR will learn how to combine the deep-UV approach with a conventional fluorescence labelling and detection using fluorescent dyes in the red spectral range. This multimodal approach will bring additional information without impairing the UV detection. Expected Results: Controlled single protein translocation through the nanopore detected label-free in the deep-UV and confirmed by red fluorescent dye signal. Influence of the UV laser on the protein conformation and potential structural damages. Role of energy transfer between the dyes. Demonstration of plasmonic trapping at the single protein level. Planned secondments: For the magneto-plasmonic trapping the JR will visit CIC GUNE for 2 months during year 1; For the fluorescence sensing in nanopore the JR will visit TU DELFT for 2 months during year 2; for deep-UV SERS spectroscopy the JR will visit BERLIN University for 3 months in year 3

    Additional comments

    DYNAMO project key facts: • Bringing together a unique team of 6 world-leading academic groups, at the forefront of nanoscience and single molecule sensing and manipulation, and 1 high tech company, to translate the innovations into real-world applications; • Training 10 Junior Researchers (JR) on a unique mix of experimental and computational skills at the physics/chemistry/biotechnology interface; • Enabling technological advances through the combination of enhanced optical spectroscopies, plasmonics and DNA nanotechnology. This will lead to the development of nanopore technologies with unprecedented functionality and single molecule control; • Reaching single molecule capturing and tweezing functionality in solid-state nanopore in a way that has not been possible before. This will pave the way to fascinating new discoveries into the fundamental structures of biomolecules and the interaction forces among them.

    Web site for additional job details

    https: // emploi.cnrs.fr/Offres/Doctorant/UMR7249-JERWEN-005/Default.aspx

    Required Research Experiences
  • Engineering

  • None

  • Physics

  • None

  • Technology

  • None

    Offer Requirements
  • Engineering: Master Degree or equivalent

    Physics: Master Degree or equivalent

    Technology: Master Degree or equivalent

  • FRENCH: Basic

    Contact Information
  • Organisation/Company: CNRS
  • Department: Institut Fresnel
  • Organisation Type: Public Research Institution
  • Website: https:// www. fresnel.fr
  • Country: France
  • City: MARSEILLE 13
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